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af488 egfr  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc af488 egfr
    Af488 Egfr, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/af488+egfr/EGF+Receptor+XP+Rabbit+mAb/pmc12179293-363-42-43
    Average 93 stars, based on 18 article reviews
    af488 egfr - by Bioz Stars, 2026-11
    93/100 stars

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    other:

    Article Title: TGFα controls checkpoints in CNS resident and infiltrating immune cells to promote resolution of inflammation
    Article Snippet: The purified cells were treated with medium containing DMEM/F12 + GlutaMAX (Thermo Fisher Scientific, #31331-093), 1% penicillin/streptomycin, B27 Supplement (Thermo Fisher Scientific, #12587-010), and N2 Supplement (Thermo Fisher Scientific, #17504-044).

    Article Title: TGFα controls checkpoints in CNS resident and infiltrating immune cells to promote resolution of inflammation.
    Article Snippet: The cell suspension containing mixed glial cells were cultured for 7 days at 37 °C and 5% CO2, with a change of medium every second day: DMEM supplemented with 20% FBS and 1% penicillin/streptomycin (Thermo Fisher Scientific, #15140122).



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    Key resources table
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    Key resources table

    Journal: iScience

    Article Title: CX3CR1 deficiency-induced TIL tumor restriction as a novel addition for CAR-T design in solid malignancies

    doi: 10.1016/j.isci.2023.106443

    Figure Lengend Snippet: Key resources table

    Article Snippet: CAR-T were characterized in a panel containing: EGFR AF488 (R&D Systems catalog number FAB9577G), CD4 PerCP-Cy5.5 (BD Biosciences catalog number 560650, RRID: AB_1727476 ), CX3CR1 APC (Thermo Fisher Scientific catalog number 17-6099-42, RRID: AB_11149136 ), CD28 APC-H7 (BD Biosciences catalog number 561368, RRID: AB_11154032 ), PD1 BV421 (BD Biosciences catalog number 562516, RRID: AB_11153482 ), CD127 BV605 (BD Biosciences catalog number 562662, RRID: AB_2737706 ), CD62L BV711 (BD Biosciences catalog number 565040, RRID: AB_2869642 ), Tim3 BV786 (BD Biosciences catalog number 742857, RRID: AB_2741100 ), CCR7 BUV395 (Thermo Fisher Scientific catalog number 17-6099-42, RRID: AB_11149136 ), TIGIT APC-Fire750 (BioLegend catalog number 372708, RRID: AB_2632755 ), CTLA-4 PE-CF594 (BD Biosciences catalog number 562742, RRID: AB_2737761 ), CD8 BUV496 (BD Biosciences catalog number 564804, RRID: AB_2744460 ), CD27 BUV 737 (BD Biosciences catalog number 564301, RRID: AB_2744350 ), CD3 BUV605 (BD Biosciences catalog number 564712, RRID: AB_2738908 ), LAG3 APC-R700 (BD Biosciences catalog number 565774, RRID: AB_2744329 ), CD95 PE-CF594 (BD Biosciences catalog number 562395, RRID: AB_11153666 ), and CD45RA PE-Cy7 (BD Biosciences catalog number 560675, RRID: AB_1727498 ).

    Techniques: Marker, Recombinant, Enzyme-linked Immunosorbent Assay, Cell Isolation, Activation Assay, Staining, Over Expression, Construct, Plasmid Preparation, Software, Microscopy, Imaging, Flow Cytometry